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Transfection mixtures 45 were prepared by combining a total of 1 μg plasmid DNA (comprising both GPX4-pTT5 and SBP2-pTT5 plasmids) for each 1 mL of transfected cell culture with PEI transfection reagent (polyethylenimine, linear, Polysciences # 23966) at a ratio of 1:2 (w/w)

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Factors That Influence Your Ideal Dose Your Body Weight: Larger individuals may require more glutathione to achieve the same cellular saturation as smaller individuals